LoadExperiment: Difference between revisions

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LoadExperiment enables you to load a set of experimental quantitative, polymorphism, or alignment data for a genome in CoGe.  Several different file formats are supported. The data can then be viewed alongside annotation in [[GenomeView]].
#REDIRECT: [[LoadExp+]]
 
== Inputs  ==
 
=== Metadata ===
*'''Name:''' Name of experiment
*'''Description:''' Description of experiment
*'''Version:''' Version of experiment
*'''Source:''' Where is the data from? This could be you, your lab, your university, a sequencing center, your collaborator.
*'''Restricted:''' Is this experiment public or restricted to you and your collaborators
*'''Genome:''' Select the appropriate genome from CoGe
*'''Select Data File:''' Opens a window for specifying the input data file
 
=== Data File  ===
 
You can select and retrieve data file located at:
 
*The iPlant Data Store
*An FTP server
*Your computer (Upload)<br>
 
=== Data Formats  ===
 
LoadExperiment supports several data file formats depending on the data type:
 
*Quantitative data
**Comma-separated (CSV) file format
**Tab-separated (TSV) file format
**BED file format
*Polymorphism (SNP) data
**Variant Call Format (VCF) file format
*Alignment data
**BAM file format
 
==== CSV File Format  ====
 
This is a comma-delimited file that contains the following columns
 
*Chromosome (string)
*Start position (integer)
*Stop position (integer)
*Chromosome Strand (1 or -1)
*Measurement Value must be between [1-0] (real number; inclusive)
*Second Value (OPTIONAL): can store a second value such as an expect value (real number)
 
#CHR,START,STOP,STRAND,VALUE1(0-1),VALUE2(ANY-ANY)
Chr1,11486,12316,1,0.181430277220112,7.3980806218146
Chr1,27309,28272,1,0.944373742485446,5.08225285439412
Chr1,32484,32978,1,0.328500324191726,1.97719838086201
Chr1,41942,42508,-1,0.825027233105203,6.56057592312617
Chr1,56394,57527,-1,0.183234367788511,0.795527328556531
Chr1,67705,68809,-1,0.956523086778851,5.20992343466606
Chr1,71144,72409,1,0.42955128220331,1.80604269639474
Chr1,81671,82833,1,0.626003507696723,2.77834108023821
Chr1,86467,87623,-1,0.0878653961575928,7.42843749315945
 
==== TSV File Format ====
 
Same as CSV format but with tab delimiters instead of commas.
 
==== BED File Format ====
 
Standard BED format as defined here:
http://genome.ucsc.edu/FAQ/FAQformat.html#format1
 
Only the first six columns are used, with the "name" field ignored.

Latest revision as of 16:37, 10 May 2017

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