Difference between revisions of "Ambiguous nucleotide"
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Ambiguous nucleotides are used when the true nucleotide is unknown. The most popular one seen with high-throughput genome sequences is "N" which represents any possible nucleotide. | Ambiguous nucleotides are used when the true nucleotide is unknown. The most popular one seen with high-throughput genome sequences is "N" which represents any possible nucleotide. | ||
| − | + | ==Ambiguous== | |
| − | * | + | *'''N''' Any nucleotide (A or C or G or T or U) |
| − | + | *'''R''' Purine (A or G) | |
| − | + | *'''Y''' Pyrimidine (T or C) | |
| − | + | *'''K''' Keto (G or T) | |
| − | * | + | *'''M''' Amino (A or C) |
| − | + | *'''S''' Strong interaction (3 H bonds) (G or C) | |
| − | *Pyrimidine (T or C) | + | *'''W''' Weak interaction (2 H bonds) (A or T) |
| − | *Keto (G or T) | + | *'''B''' Not A (C or G or T) |
| − | *Amino (A or C) | + | *'''D''' Not C (A or G or T) |
| − | *Strong interaction (3 H bonds) (G or C) | + | *'''H''' Not G (A or C or T) |
| − | *Weak interaction (2 H bonds) (A or T) | + | *'''V''' Not T or U (A or C or G) |
| − | *Not A (C or G or T) | + | |
| − | *Not C (A or G or T) | + | |
| − | *Not G (A or C or T) | + | |
| − | *Not T or U (A or C or G) | + | |
| − | *''' | + | ==Not ambiguous== |
| + | * '''A''' Adenosine | ||
| + | * '''C''' Cytidine | ||
| + | *'''G''' Guanine | ||
| + | *'''T''' Thymidine | ||
| + | *'''U''' Uridine (if 'Alphabet' set to 'RNA') | ||
Latest revision as of 17:09, 23 June 2011
Ambiguous nucleotides are used when the true nucleotide is unknown. The most popular one seen with high-throughput genome sequences is "N" which represents any possible nucleotide.
Ambiguous
- N Any nucleotide (A or C or G or T or U)
- R Purine (A or G)
- Y Pyrimidine (T or C)
- K Keto (G or T)
- M Amino (A or C)
- S Strong interaction (3 H bonds) (G or C)
- W Weak interaction (2 H bonds) (A or T)
- B Not A (C or G or T)
- D Not C (A or G or T)
- H Not G (A or C or T)
- V Not T or U (A or C or G)
Not ambiguous
- A Adenosine
- C Cytidine
- G Guanine
- T Thymidine
- U Uridine (if 'Alphabet' set to 'RNA')
