Difference between revisions of "Ambiguous nucleotide"

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(Ambiguous)
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==Ambiguous==
 
==Ambiguous==
*Any nucleotide (A or C or G or T or U) '''N'''
+
*'''N'''  Any nucleotide (A or C or G or T or U)
*Purine (A or G) '''R'''  
+
*'''R''' Purine (A or G)
*Pyrimidine (T or C) '''Y'''  
+
*'''Y''' Pyrimidine (T or C)  
*Keto (G or T) '''K'''  
+
*'''K''' Keto (G or T)  
*Amino (A or C) '''M'''  
+
*'''M''' Amino (A or C)  
*Strong interaction (3 H bonds) (G or C) '''S'''  
+
*'''S''' Strong interaction (3 H bonds) (G or C)  
*Weak interaction (2 H bonds) (A or T) '''W'''  
+
*'''W''' Weak interaction (2 H bonds) (A or T)  
*Not A (C or G or T) '''B'''  
+
*'''B''' Not A (C or G or T)  
*Not C (A or G or T) '''D'''  
+
*'''D''' Not C (A or G or T)
*Not G (A or C or T) '''H'''  
+
*'''H''' Not G (A or C or T)  
*Not T or U (A or C or G) '''V'''
+
*'''V''' Not T or U (A or C or G)
 +
 
 
==Not ambiguous==
 
==Not ambiguous==
 
*Adenosine  '''A'''  
 
*Adenosine  '''A'''  

Revision as of 17:09, 23 June 2011

Ambiguous nucleotides are used when the true nucleotide is unknown. The most popular one seen with high-throughput genome sequences is "N" which represents any possible nucleotide.

Ambiguous

  • N Any nucleotide (A or C or G or T or U)
  • R Purine (A or G)
  • Y Pyrimidine (T or C)
  • K Keto (G or T)
  • M Amino (A or C)
  • S Strong interaction (3 H bonds) (G or C)
  • W Weak interaction (2 H bonds) (A or T)
  • B Not A (C or G or T)
  • D Not C (A or G or T)
  • H Not G (A or C or T)
  • V Not T or U (A or C or G)

Not ambiguous

  • Adenosine A
  • Cytidine C
  • Guanine G
  • Thymidine T
  • Uridine (if 'Alphabet' set to 'RNA') U