Jcvi code

Background

Rumor has it that there is a code in one of the synthetic genomes by JCVI. Supposedly, this code contains an email address or a URL (or a secret message from Dr. V!).

Synthetic JCVI genomes in CoGe

• synthetic Mycoplasma genitalium strain JCVI-1.0: http://genomevolution.org/CoGe/OrganismView.pl?oid=35986
• synthetic Mycoplasma mycoides JCVI-syn1.0: http://genomevolution.org/CoGe/OrganismView.pl?oid=35385

Methods

1. Find closest natural relatives
2. Identify syntenic discontinuities (this is where the new JCVI code should reside
3. Decode new sequence
   1. Identify coding scheme
      1. Probably using natural codon triplet encoding given that:
         1. 1x4 encoding = 4 letters
         2. 2x4 encoding = 16 letters
         3. 3x4 encoding = 64 letters
      2. Given that there are 20ish natural amino acids, some of the codons will be appropriated for additional letters and symbols
         1. An example for students of expanded codon encoding (using neighboring codons for additional letters): http://nature.ca/genome/05/051/0511/0511_m205_e.cfm
   2. Decode email address
4. Valid email address

Closest natural relatives

Syntenic dotplot of synthetic Mycoplasma genitalium strain JCVI-1.0 (y-axis) v. Mycoplasma genitalium strain G37 (x-axis) http://genomevolution.org/r/4mx1	Syntenic dotplot of synthetic Mycoplasma mycoides JCVI-syn1.0 (y-axis) v. Mycoplasma mycoides subsp. capri strain GM12 (x-axis) http://genomevolution.org/r/4mx2

GEvo Analyses: high-resolution detection of syntenic discontinuities

Genitalium

GEvo whole genome analysis of Mycoplasma genitalium strain JCVI-1.0 v. Mycoplasma genitalium strain G37. Results may be regenerated at: http://genomevolution.org/r/4mwy

GEvo analysis of syntenic discontinuity ofMycoplasma genitalium strain JCVI-1.0 v. Mycoplasma genitalium strain G37. http://genomevolution.org/r/4mx3

• Disrupted WT gene: MG_408 , NP_073081.1 , pmsR
  • methionine sulfoxide reductase A
  • this stereospecific enzymes reduces the S isomer of methionine sulfoxide while MsrB reduces the R form a fusion protein of this enzyme with MsrB provides protection against oxidative stress in Neisseria gonorrhoeae this stereospecific enzymes reduces the S isomer of methionine sulfoxide while MsrB reduces the R form
• Inserted gene: ABY79711.1 , MGATCC33530_0530
  • bifunctional AAC/APH (AAC(6'): 6'-aminoglycoside N-acetyltransferase and APH(2'): 2-aminoglycoside phosphotransferase
  • Aminoglycoside antibiotic resistance is largely the result of the production of enzymes that covalently modify the drugs including kinases (Aph) with structural and functional similarity to protein and lipid kinases. One of the most important aminoglycoside resistance enzymes is Aac(6')-Aph(2), a bifunctional enzyme with both aminoglycoside acetyltransferase and kinase activities.
• Extract sequence: http://genomevolution.org/CoGe//SeqView.pl?featid=143193835_1&dsid=63956&chr=1&start=&upstream=-227770&downstream=228068&rc=0&dsgid=15768
  • Perform 6-frame translation (using above link)
  • search for anything that might match "com/cnm" "org/nrg" "net", "cvi" (jcvi)
    • Nothing: could be wrong code

Mycoides

GEvo whole genome analysis of Mycoplasma mycoides JCVI-syn1.0 v. Mycoplasma mycoides subsp. capri strain GM12. Results may be regenerated at http://genomevolution.org/r/4mxd

Region	Notes
http://genomevolution.org/r/4mxl	Annotation talks about how a piece of e. coli made it into this area. Not the greener region (GC rich). When that sequence is extracted and blasted against E. coli, get a very high quality hit (95%). http://genomevolution.org/CoGe//CoGeBlast.pl?chr=contig_sMmYCp235-1&upstream=308819&downstream=309545&dsid=59166&rc=0&gstid=1
http://genomevolution.org/r/4mxq	http://genomevolution.org/CoGe//SeqView.pl?dsid=59166&chr=contig_sMmYCp235-1&start=389504&stop=390368&gstid=1. There is an obvious "new thing" that is annotated as a hypothetical protein predicted by glimmer. Gene it replaces is a transposon (which wouldn't be missed). This sequence does not match anything in K12 MG1655. Its sequence, when 6-frame translated, yields one frame that contains several "CVI" amino acid sequences. This sequence hasn't been seen in other aa sequences from this genome. Obvious email/web address did not jump out.
http://genomevolution.org/r/4myz	http://genomevolution.org/CoGe//SeqView.pl?dsid=59166&chr=contig_sMmYCp235-1&start=565568&stop=566608&gstid=1 . Did not match anything in E. coli. No obvious words. Disrupted (removed) WT ABC transporters.
http://genomevolution.org/r/4mzj	http://genomevolution.org/CoGe//SeqView.pl?dsid=59166&chr=contig_sMmYCp235-1&start=725610&stop=726650&gstid=1 . Did not match anything in E. coli. Did contain VCI. Interrupted WT "Type III restriction-modification system is named MmyCI"
http://genomevolution.org/r/4mzp	http://genomevolution.org/CoGe//SeqView.pl?dsid=59166&chr=contig_sMmYCp235-1&start=958651&stop=959707&gstid=1 . Did not match anything in E. coli. Did contain VCI. Interrupting a WT transposon. Hit nothing else in NCBI NR database.

Hints From the Paper and Craig V.

The paper describes four watermark sequences added to the genome. CoGe correctly identified these (along with the carryover piece of E. coli). There is a video and associated articles from Craig describing that the four watermarks contain special phrases. One of which is the URL/email address.

1. Paper: http://www.sciencemag.org/content/329/5987/52.abstract
2. Sup Data: http://www.sciencemag.org/content/suppl/2010/05/18/science.1190719.DC1/Gibson.SOM.pdf
3. http://www.popsci.com/science/article/2010-05/venter-institutes-synthetic-cell-genome-contains-hidden-messages-watermarks
4. http://en.wikipedia.org/wiki/Mycoplasma_laboratorium
5. JCVI code phrases
6. JCVI watermarks (without end sequences)
7. Aligned 6-frame translation of watermark 4 shows nothing that matches phrase 4

Decoding part one

The code is something different than using protein translation. Also, there needs to be a way to code special characters, such as "," or ":", etc.

JCVI GEvo analysis of watermarks JCVI notes

Cracking the code (from the repeat sequences)

ATA AAC CTG GGC TAA
<s> l   i   f   e

TGA ATA TAG GCT ATA TGA TCA TAA CAT ATA
t   <s> a   s   <s> t   h   e   y   <s>

ATA CTG ATA TTT TAG TGC TGC CGT TGA ATA
<s> I   <s> c   a   n   n   o   t   <s>
:)

1. Notes:
   1. spaces all use the same triplet (ATA)
   2. lower case "i" and upper-case "I" both use the same triplet (CTG): code is case insensitive