Difference between revisions of "Ambiguous nucleotide"

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Ambiguous nucleotides are used when the true nucleotide is unknown. The most popular one seen with high-throughput genome sequences is "N" which represents any possible nucleotide.  
 
Ambiguous nucleotides are used when the true nucleotide is unknown. The most popular one seen with high-throughput genome sequences is "N" which represents any possible nucleotide.  
  
*Nucleotide Code
+
*Any nucleotide (A or C or G or T or U) '''N'''
 
*Adenosine  '''A'''  
 
*Adenosine  '''A'''  
 
*Cytidine '''C'''  
 
*Cytidine '''C'''  
*Guanine G  
+
*Guanine '''G'''
*Thymidine T  
+
*Thymidine '''T'''
*Uridine (if 'Alphabet' set to 'RNA') U  
+
*Uridine (if 'Alphabet' set to 'RNA') '''U'''
*Purine (A or G) R  
+
*Purine (A or G) '''R'''
*Pyrimidine (T or C) Y  
+
*Pyrimidine (T or C) '''Y'''
*Keto (G or T) K  
+
*Keto (G or T) '''K'''
*Amino (A or C) M  
+
*Amino (A or C) '''M'''
*Strong interaction (3 H bonds) (G or C) S  
+
*Strong interaction (3 H bonds) (G or C) '''S'''
*Weak interaction (2 H bonds) (A or T) W  
+
*Weak interaction (2 H bonds) (A or T) '''W'''
*Not A (C or G or T) B  
+
*Not A (C or G or T) '''B'''
*Not C (A or G or T) D  
+
*Not C (A or G or T) '''D'''
*Not G (A or C or T) H  
+
*Not G (A or C or T) '''H'''
*Not T or U (A or C or G) V
+
*Not T or U (A or C or G) '''V'''
 
+
*'''Any nucleotide (A or C or G or T or U) N'''
+

Revision as of 17:07, 23 June 2011

Ambiguous nucleotides are used when the true nucleotide is unknown. The most popular one seen with high-throughput genome sequences is "N" which represents any possible nucleotide.

  • Any nucleotide (A or C or G or T or U) N
  • Adenosine A
  • Cytidine C
  • Guanine G
  • Thymidine T
  • Uridine (if 'Alphabet' set to 'RNA') U
  • Purine (A or G) R
  • Pyrimidine (T or C) Y
  • Keto (G or T) K
  • Amino (A or C) M
  • Strong interaction (3 H bonds) (G or C) S
  • Weak interaction (2 H bonds) (A or T) W
  • Not A (C or G or T) B
  • Not C (A or G or T) D
  • Not G (A or C or T) H
  • Not T or U (A or C or G) V