Difference between revisions of "Ambiguous nucleotide"

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Ambiguous nucleotides are used when the true nucleotide is unknown. The most popular one seen with high-throughput genome sequences is "N" which represents any possible nucleotide.  
 
Ambiguous nucleotides are used when the true nucleotide is unknown. The most popular one seen with high-throughput genome sequences is "N" which represents any possible nucleotide.  
  
 +
==Ambiguous==
 
*Any nucleotide (A or C or G or T or U) '''N'''
 
*Any nucleotide (A or C or G or T or U) '''N'''
*Adenosine  '''A'''
 
*Cytidine '''C'''
 
*Guanine '''G'''
 
*Thymidine '''T'''
 
*Uridine (if 'Alphabet' set to 'RNA') '''U'''
 
 
*Purine (A or G) '''R'''  
 
*Purine (A or G) '''R'''  
 
*Pyrimidine (T or C) '''Y'''  
 
*Pyrimidine (T or C) '''Y'''  
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*Not G (A or C or T) '''H'''  
 
*Not G (A or C or T) '''H'''  
 
*Not T or U (A or C or G) '''V'''
 
*Not T or U (A or C or G) '''V'''
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==Not ambiguous==
 +
*Adenosine  '''A'''
 +
*Cytidine '''C'''
 +
*Guanine '''G'''
 +
*Thymidine '''T'''
 +
*Uridine (if 'Alphabet' set to 'RNA') '''U'''

Revision as of 17:07, 23 June 2011

Ambiguous nucleotides are used when the true nucleotide is unknown. The most popular one seen with high-throughput genome sequences is "N" which represents any possible nucleotide.

Ambiguous

  • Any nucleotide (A or C or G or T or U) N
  • Purine (A or G) R
  • Pyrimidine (T or C) Y
  • Keto (G or T) K
  • Amino (A or C) M
  • Strong interaction (3 H bonds) (G or C) S
  • Weak interaction (2 H bonds) (A or T) W
  • Not A (C or G or T) B
  • Not C (A or G or T) D
  • Not G (A or C or T) H
  • Not T or U (A or C or G) V

Not ambiguous

  • Adenosine A
  • Cytidine C
  • Guanine G
  • Thymidine T
  • Uridine (if 'Alphabet' set to 'RNA') U