Fish Comparative Genomics: Difference between revisions

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== Setting the hook: getting data loaded into CoGe ==  
== Setting the hook: getting data loaded into CoGe ==
 
*Loading new genomes into CoGe  
*Loading new genomes into CoGe  
**Loading from the iPlant Data Store When loading a new genome  
**Loading from the iPlant Data Store When loading a new genome  
**Using FTP/HTTP links for uploading genomes  
**Using FTP/HTTP links for uploading genomes  
**Direct upload  
**Direct upload  
**NCBI Loader <br>  
**NCBI Loader <br>
 
 
*Table of fish genomes publicly available in CoGe


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*Table of fish genomes publicly available in CoGe


{| width="1075" cellspacing="1" cellpadding="1" border="1" height="1387" |- | <b>Class</b> | <b>Order</b>    | <b>Genus species  </b> | <b>Common name</b>    | <b>Notes</b>    | <b>CoGe Genome Links</b> |- | Cephalaspidomorphi    | Petromyzontiformes    | Lethenteron camtschaticum    | Artic lamprey    | One of few extant jawless fish    | |- |    |    | Petromyzon marinus    | Sea lamprey    | One of few extant jawless fish    | |- | Chondrichthyes    | Chimaeriformes    | Callorhinchus milii    | Australian ghostshark    | Proposed model cartilaginous fish    | |- | Sarcopterygii    | Coelacanthiformes    | Latimeria chalumnae    | Coelacanth    | Oldest known extant Sarcopterygii    | |- | Actinopterygii    | Anguilliformes    | Anguilla japonica    | Japanese eel    | Ecological model, Industry species    | |- |    | Beloniformes    | Oryzias latipes    | Medaka    | Model fish, Japanese pet fish    | |- |    | Characiformes    | Astyanax mexicanus    | Mexican tetra    | Two forms: seeing, and cave-dwelling blind    | |- |    | Cichliformes    | Haplochromis burtoni    | Burton’s mouthbrooder    | Ecological model species, Aquarium species    | |- |    |    | Labeotropheus fuelleborni    | Blue mbuna    |    | |- |    |    | Maylandia zebra    | Zebra mbuna    | Ecological model species, aquarium fish    | |- |    |    | Mchenga conophoros    |    |    | |- |    |    | Melanochromis auratus    | Auratus cichlid    | Phenotypic and ecological diversity    | |- |    |    | Neolamprologus brichardi    | Princess cichlid    | Aquarium fish    | |- |    |    | Oreochromis niloticus    | Nile tilapia | Industrial species    | |- |    |    | Pundamilia nyererei    |    |    | |- |    | Cypriniformes    | Danio rerio    | Zebrafish    | Model species    | |- |    |    | Pimephales promelas    | Fathead minnow    | Industrial baitfish    | |- |    | Cyprinodontiformes    | Cyprinodon variegatus    | Sheepshead minnow    | Toxicology model    | |- |    |    | Nothobranchius furzeri    | Turquoise killifish    | Short life span model species, metabolic diapause    | |- |    |    | Nothobranchius kuhntae    | Beira killifish    | Short life model species    | |- |    |    | Poecilia formosa    | Amazon molly    | Gynogenesis: all female populations    | |- |    |    | Poecilia reticulata    | guppy    | Model species, aquarium fish    | |- |    |    | Xiphophorus maculatus    | Southern platyfish    | Gives live birth    | |- |    | Esociformes    | Esox lucius    | Northern pike    | Angling species    | |- |    | Gadiformes    | Gadus morhua    | Atlantic cod    | Industrial species    | |- |    | Perciformes    | Anoplopoma fimbria    | Sablefish    | Industrial species    | |- |    |    | Dicentrarchus labrax    | European seabass    | Industrial species    | |- |    |    | Gasterosteus aculeatus    | Three-spined stickleback    | Model species    | |- |    |    | Sebastes nigrocinctus    | Tiger rockfish    | Angling species, long lived model, live bearer    | |- |    |    | Sebastes rubrivinctus    | Flag rockfish    | Angling species, industrial species    | |- |    |    | Stegastes partitus    | Bicolor damselfish    | Medical model species    | |- |    | Pleuronectiformes    | Cynoglossus semilaevis    | Tongue sole    | Industrial species    | |- |    | Salmoniformes    | Oncorhyncus mykiss    | Rainbow trout    | Angling species, Indrustrial species    | |- |    | Semionotiformes    | Lepisosteus oculatus    | Spotted gar    |    | |- |    | Scombriformes    | Thunnus orientalis    | Pacific bluefin tuna    | Industrial species    | |- |    | Tetraodontiformes    | Takifugu flavidus    | Sansaifugu    |    | |- |    |    | Tetraodon nigroviridis    | Spotted green pufferfish    | Model species, low amount of repetitive sequence    | |- |    |    | Takifugu rubripes    | ‘Fugu’ pufferfish    | Shortest vertebrate genome  | |}   


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*Links to CoGe [[Notebooks]] containing these genomes<br>  
*Links to CoGe [[Notebooks]] containing these genomes<br>  

Revision as of 22:14, 5 December 2014

Summary/Abstract

Introduction

  • Phylogeny of genomes with polyploidy events marked




Setting the hook: getting data loaded into CoGe

  • Loading new genomes into CoGe
    • Loading from the iPlant Data Store When loading a new genome
    • Using FTP/HTTP links for uploading genomes
    • Direct upload
    • NCBI Loader


  • Table of fish genomes publicly available in CoGe



Glossary

Analyses

Whole genome syntenic analysis

  • SynMap
    • Identify Whole Genome Duplications
    • Identify Synteny
    • Synonymous/nonsynonymous gene pair evolution

Microsyntenic analysis

  • GEvo
    • Validate microsynteny
    • Identify Conserved non-coding sequences (regulatory function)

Ortholog/paralog finding with synteny

  • SynFind
    • Identify orthologous regions across many species

Gene family analysis

  • CoGeBlast
    • Identify many gene family members within/across species
    • Extract sequences (nucleotide/protein)
    • Phylogenetic tree reconstruction using iPlant/iAnimal for multiple sequence alignment and tree building

Functional genomics

  • Adding/visualizing RNAseq data

Adding new data (genomes, RNASeq, SNPs) to CoGe

Discussion/Conclusions