Ambiguous nucleotide
From CoGepedia
Ambiguous nucleotides are used when the true nucleotide is unknown. The most popular one seen with high-throughput genome sequences is "N" which represents any possible nucleotide.
Ambiguous
- N Any nucleotide (A or C or G or T or U)
- R Purine (A or G)
- Y Pyrimidine (T or C)
- K Keto (G or T)
- M Amino (A or C)
- S Strong interaction (3 H bonds) (G or C)
- W Weak interaction (2 H bonds) (A or T)
- B Not A (C or G or T)
- D Not C (A or G or T)
- H Not G (A or C or T)
- V Not T or U (A or C or G)
Not ambiguous
- Adenosine A
- Cytidine C
- Guanine G
- Thymidine T
- Uridine (if 'Alphabet' set to 'RNA') U
