2015 PAG Computer Demo
Slides
- Keynote:
- PDF:
Register an account/Log in
- Go to: http://user.iplantcollaborative.org
- CoGe uses iPlant's Authentication and User Identify Management Service
- After clicking on the confirmation link provided in the automated email, your account may take a few minutes to propagate to all of iPlant's Authentication Services.
- Sign-in (link is in top-right of any CoGe page)
- NOTE: This wiki (CoGePedia) uses a different authentication than CoGe!
Load your own genome
- Start:
- From your user profile page (must be logged into CoGe): https://genomevolution.org/CoGe/User.pl
- Click "Create"-> "New Genome"
- Go directly to LoadGenome: https://genomevolution.org/CoGe/LoadGenome.pl
- From your user profile page (must be logged into CoGe): https://genomevolution.org/CoGe/User.pl
- Search for Organism "Escherichia coli K12 strain K-12 substrain MG1655" (just type in "MG1655")
- Set a version (e.g., "1")
- Leave "Type:" as "unmasked"
- Source: "CoGe" or "NCBI"
- Leave as "Restricted"
- Press "Add Data"
- Select "FTP/HTTP" tab
- Paste in the link below:
- Press "Get"
- When finished being retrieved, press "Load Genome"
- Note: The length of time it takes to load a genome depends on the load on the database and the number of chromosomes/contigs being loaded. For this example, it should take a minute or two.
Add Annotations
- Go to GenomeInfo by pressing "View Genome"
- Press "Load Gene Annotations"
- Set a version (e.g., "1")
- Source: "CoGe" or "NCBI"
- Leave Restricted
- Press "Select Data Files"
- Select "FTP/HTTP" Tab
- Paste in link below:
- E. coli annotations: http://de.iplantcollaborative.org/dl/d/C1ACC5C3-1E0B-409B-8A91-C969943F41F8/7122-structural-annotations.gff
- Press "Get"
- When finished being retrieved, press "Load Annotations"
- Note: The length of time it takes to load annotations depends on the load on the database and the number of annotations being loaded. For this example (and no load on the server), it should take ~ 5 minutes.
Comparative Genomics
- Human-Chimp whole genome: http://genomevolution.org/r/d76i
- Arabidopsis thaliana self-self: http://genomevolution.org/r/d7p5
- Arabidopsis thaliana v. Arabidopsis lyrata: http://genomevolution.org/r/d4hi
- Arabidopsis thaliana v. Arabidopsis lyrata (Synonymous values): http://genomevolution.org/r/d7e7
- SynFind
- Find a peach gene: https://genomevolution.org/CoGe/GenomeView.pl?chr=scaffold_1&gid=22743&x=10266537&z=6&loc=scaffold_1%3A10266380..10666380&tracks=sequence%2Cfeatures
- Send to SynFind: https://genomevolution.org/CoGe/SynFind.pl?fid=461399390
- Search genome of Arabidopsis thaliana: http://genomevolution.org/r/d774
- Microsynteny analysis: http://genomevolution.org/r/d778
Loading your own genome
- E. coli genome: http://de.iplantcollaborative.org/dl/d/555B53F9-E738-4951-85A3-421E23804DFA/genome_7112.faa
- E. coli annotations: http://de.iplantcollaborative.org/dl/d/C1ACC5C3-1E0B-409B-8A91-C969943F41F8/7122-structural-annotations.gff
- E. coli expression data: http://de.iplantcollaborative.org/dl/d/F1EE1A86-F91A-46E7-92D6-A25EE78E3DA3/7112-lo.csv
- E. coli variant data: http://de.iplantcollaborative.org/dl/d/B4040AC9-74A7-4E3E-B074-F23E1D70823E/7112-demo.vcf.gz
- E. coli bam alignment data: http://de.iplantcollaborative.org/dl/d/D0FC54E4-6DA6-4604-BE19-81F1D33B5350/7112-alignment.bam
RNASeq Processing
- Arabidopsis RNA-Seq data: http://de.iplantcollaborative.org/dl/d/A7F5E57F-E776-46E0-9672-59264E663F8A/test_rna_seq_data_0.17M_reads.fastq
CoGe Learning Material
- Loading private data in CoGe: http://www.youtube.com/watch?v=JPZ8IrPnh_8
- RNASeq with EPIC-CoGe: https://www.youtube.com/watch?v=3fNyHGB02dM
- Tutorials: https://genomevolution.org/wiki/index.php/Tutorials
- Arabidopsis/Brassica: http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00172/full
- Grasses (maize/sorghum): http://www.maydica.org/articles/56_183.pdf