Difference between revisions of "2015 Plant Genome Evolution Workshop"

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(Medium Genome)
(Small Genome)
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* Press "Next"
 
* Press "Next"
 
* Review the data and associated information.   
 
* Review the data and associated information.   
* Press "Start Loading"
+
* Press ''"Start Loading"''
 
* Note: The length of time it takes to load a genome depends on the load on the database and the number of chromosomes/contigs being loaded.  For this example, it should take a minute or two.
 
* Note: The length of time it takes to load a genome depends on the load on the database and the number of chromosomes/contigs being loaded.  For this example, it should take a minute or two.
 +
* Note: When finished, you can select what you want to do next from a drop-down menu:
 +
** Go to [[GenomeInfo]]
 +
** Load Annotations for the genome
 +
** Load Another Genome
  
 
====Medium Genome====
 
====Medium Genome====

Revision as of 16:35, 26 August 2015

Slides

  • Keynote:
  • PDF:
  • Powerpoint:

Register an account/Log in

  • Go to: http://user.iplantcollaborative.org
    • CoGe uses iPlant's Authentication and User Identify Management Service
    • After clicking on the confirmation link provided in the automated email, your account may take a few minutes to propagate to all of iPlant's Authentication Services.
  • Sign-in (link is in top-right of any CoGe page)
  • NOTE: This wiki (CoGePedia) uses a different authentication than CoGe!

Load your own genome

Small Genome

  • Search for Organism "Escherichia coli K12 strain K-12 substrain MG1655" (just type in "MG1655")
  • Set a version (e.g., "1")
  • Leave "Type:" as "unmasked"
  • Source: "CoGe" or "NCBI"
  • Leave as "Restricted"
  • Press "Next"
  • Select "FTP/HTTP" tab
  • Paste in the link below:
  • Press "Get"
  • Press "Next"
  • Review the data and associated information.
  • Press "Start Loading"
  • Note: The length of time it takes to load a genome depends on the load on the database and the number of chromosomes/contigs being loaded. For this example, it should take a minute or two.
  • Note: When finished, you can select what you want to do next from a drop-down menu:
    • Go to GenomeInfo
    • Load Annotations for the genome
    • Load Another Genome

Medium Genome

  • Search for Organism "Arabidopsis thaliana Col-0 (thale cress)" (just type in "col-0")
  • Set a version (e.g., "1")
  • Leave "Type:" as "unmasked"
  • Source: "CoGe" or "TAIR"
  • Leave as "Restricted"
  • Press "Next"
  • Select "FTP/HTTP" tab
  • Paste in the link below:
  • Press "Get"
  • Press "Next"
  • Review the data and associated information.
  • Press "Start Loading"
  • Note: The length of time it takes to load a genome depends on the load on the database and the number of chromosomes/contigs being loaded. For this example, it should take a minute or two.
  • Note: When finished, you can select what you want to do next from a drop-down menu:
    • Go to GenomeInfo
    • Load Annotations for the genome
    • Load Another Genome

Add Annotations

  • Go to GenomeInfo by pressing "View Genome"
  • Press "Load Gene Annotations"
  • Set a version (e.g., "1")
  • Source: "CoGe" or "NCBI"
  • Leave Restricted
  • Press "Select Data Files"
  • Select "FTP/HTTP" Tab
  • Paste in link below:
  • When the load is finished, pressing "Genome View" will launch the genome viewer (JBrowse).
  • Note: The length of time it takes to load annotations depends on the load on the database and the number of annotations being loaded. For this example (and no load on the server), it should take ~ 3-5 minutes.

Find and visualize a genome



Data Management

  • Go to your User Profile page: https://genomevolution.org/CoGe/User.pl
  • Select a genome by clicking on it.
  • Information about the genome will appear in the right panel
  • You can share a genome by clicking on the person icon
  • You can delete the genome by clicking on the trash can
  • Double-clicking the genome will open the Detailed View for it (GenomeInfo)
  • Share it with the person next to you
  • You can view genomes (and other data) that has been shared with you by clicking on "Shared with me" in the menu on the left.

Analyses

  • Get the detailed view of your genome (GenomeInfo)
  • Under "Tools" and next to "Analyze", click on the link for "SynMap"
  • Your genome will automatically be populated for both genomes
  • Search for another E. coli genome by typing "MG1655" into one of the Organism search boxes
    • The one auto-select from that search will be perfect for the analysis (ID 4242)
  • Scroll to the bottom of the page and press the red button "Generate SynMap"
  • When the analysis is finished, press "Go" to see the results
  • Click on the dotpot to get a zoomed-in version of the dotplot.
  • Scroll onto the green line in the dotplot and double click when the cross-hairs turn red to launch GEvo for microsynteny analysis
  • Press "Run GEvo" to run GEvo
  • Note: This link will run a SynMap analysis for E. coli K12 substrains MG1655 and DH10B: https://genomevolution.org/r/daqz

Your History and Activity

  • If you are logged into CoGe, CoGe will record your activities. These are available for review in your User Profile page: https://genomevolution.org/CoGe/User.pl
  • Click on "Activity" in the menu on the left. This will give you an overview of the number of analyses you've run
  • Your previously run analyses can be viewed by clicking "Analyses". Clicking on an analysis will re-run it.
  • Your previously loaded data can be viewed by clicking "Data loading". Clicking on a previously loaded data will open the detailed view for those data.

Adding Experimental Data

Comparative Genomics

  • Arabidopsis thaliana v. Arabidopsis lyrata (Synonymous values): http://genomevolution.org/r/d7e7
    • Go to SynMap: https://genomevolution.org/CoGe/SynMap.pl
    • Search for "Col-0" and "lyrata" in each of the Organism search boxes.
    • Select the "Analysis Options" tab near the top of the screen
    • Under "CodeML" and next to "Calculate syntenic CDS pairs and color dots", select "Synonymous (Ks)"
    • Press "Generate SynMap"

RNASeq Processing

  • Note: You can add private experimental data to public genomes (Mix and match public and private data)
  • Go to your User Profile page: https://genomevolution.org/coge/User.pl
  • Select "Create" -> "New Experiment"
  • Add experiment name: (e.g., "RNASeq-test")
  • Add description
  • Add version
  • Add source (e.g., "coge")
  • Keep restricted
  • Search for "Col-0"
    • Make sure to select the version with genome ID 16911
  • Press "Select Data File"
  • Select the "FTP/HTTP" tab
  • Copy in the following link:
  • Press "Get"
  • It will automatically detect that it is a fastq file based on the file name extension
  • Leave the aligner set to "GSNAP" which is faster than Bowtie2
  • Press "Load Experiment"
    • Note: This Fastq file is relatively small and the whole pipeline takes around 2-3 minutes to complete
  • When finished, Load Experiment will create a notebook with three experiments
    • One for the BAM file (alignment)
    • One for reads mapped to nucleotide positions in the genome (read depth)
    • One for reads normalized to transcripts (FPKM)
  • Press "Notebook View" to view the notebook with all three experiments
  • Press "View" to visualize these data in the genome browser (JBrowse)
    • Due to the number of experiments (public) available for Arabidopsis Col-0, it may take JBrowse a while to load.

Data files reference

CoGe Learning Material