Analysis of variations found in genomes of Escherichia coli strain K12 DH10B and strain B REL606 using SynMap and GEvo analysis
- 1 Background
- 2 Generating a syntenic dotplot of two Escherichia coli strains
- 3 Syntenic dotplot data interpretation and analysis
- 4 High-resolution sequence analysis using SynMap's links to GEvo
- 5 GEvo's results
- 6 High-resolution GEvo analysis of an insertion
- 7 Detailed analysis of each syntenic discontinuity
In this exercise you will compare the genomes of two Escherichia coli strains, K12 DH10B and B REL606, using whole genome syntenic comparison and high-resolution analyses of specific genomic regions. These analyses will use CoGe's tools SynMap and GEvo respectively, and will reveal evolutionary changes between these two genomes that happened after the divergence of their lineages. While the nucleotide sequence of these genomes is identical over large expanses of their genomes, many other types of large-scale genomic change will be discovered including phage insertions, transposon transposition, and genomic insertion, deletion, inversion, and duplication events. The computational tools used to do these analyses can be used for comparing genomes of any organisms.
Generating a syntenic dotplot of two Escherichia coli strains
First, you are going to identify syntenic regions between these genomes. Synteny is defined as two or more genomic regions that share a common ancestry and thus are derived from a common ancestor. To do this, you are going to construct a Syntenic dotplot of K12 DH10B and B REL606 using SynMap. Go to SynMap Search for these E. coli strains in CoGe's database by typing in part of the their names in the "Name" search boxes for Organism 1 and Organism 2. For example, search for "DH10B" and "REL606" respectively, or type "escheri" in both boxes. Alternatively, use this link to load SynMap with these genomes already specified. Once CoGe has found organisms matches these names, make sure they are selected in the Organism List. While there are several parameters that can be configured when generating a syntenic dotplot using SynMap, the default settings work well for most situations, and very well for closely related organisms. Click "Generate SynMap" to start the analysis.
A lot of processing is happening behind the scenes, but the general way a syntenic dotplot is created is the genomes are compared to one another in order to find putative homologous genes between them, and then these pairs of genes are processed to find collinear series of genes in both genomes. The general principle is that the most likely and parsimonious way two genomes have a collinear series of homologous genes is those genomic regions in each organism are derived from a common ancestral genomic region (hence they are syntenic).
Syntenic dotplot data interpretation and analysis
When finished, SynMap will display a dotplot. Each axis of the dotplot is in nucleotide units and represents one of the two genomes laid end to end. The lower-left corner represents the start of each genome (usually 'ORI' for circular bacterial genomes), and the end of each axis is the end of each chromosome. Each putative homologous gene-pair is drawn as a gray dot on the dotplot with its position corresponding to the genomic position of each gene in their respective genomes. Gene-pairs that have been identified has being syntenic are colored green. The collection of these dots appear as green line, which for the comparison of these two genomes, in nearly continuous along the entire length of both genomes.
If you look closely at the syntenic dotplot of these genomes, you'll notice that the syntenic line is not perfect, and there are many "breaks" or discontinuities between them. In the figure above, these are indicated by a numbered arrow. These breaks in the syntenic path between these genomes are due to genomic changes happening at a larger scale than a single nucleotide polymorphism, and are mostly likely due to the insertion or deletion of a many nucleotide chunk in one of these genomes. In order to accurately account and characterize these discontinuities in the dotplot, you need to perform a high-resolution analysis of these regions using GEvo. GEvo allows you to run pairwise comparisons between multiple genomic regions where you can specify how big of a genomic region to analyze. More information on GEvo software tool can be found at: GEvo. To analyse each of these "breaks" in the sytnenic dotplot, first click on the dotplot. This will open a new window with a close-up of the dotplot. When this window appears use the cross-hair locator that appears when you mouse over the dotplot and place it on the green spot right before a "break". The locator will turn "red" when it is over a gene-pair that can be used as a link to GEvo. When the locator has turned red, click. For example, in order to visualize GEvo analysis of "break" number six, position the locator where the number six arrow points on dotplot. Click when the locator turns "red" or use this address: tinyurl.com/yl4vlbb to regenerate this particular analysis.
After positioning the locator and clicking , a new page for GEvo will appear displaying the sequence information corresponding to our region of interest in the dotplot. These sequences are "anchor" points into these two genomes for specifying the genomic regions to be compared. When linking to GEvo, SynMap automatically sets GEvo to specify using 50,000 nucleotides to the left and right of the anchor point. By default, GEvo will use BlastZ for its sequence comparison algorithm, which is a good choice for identifying large blocks of similar sequence. These settings (~100kb of each genome; BlastZ) usually work well for an initial analysis, and all you need to do is click "Run GEvo Analysis!".
Once the GEvo analysis results appear, we can begin to look for and characterize the differences between these two genomes at this syntenic region. GEvo's results will show two panels, one for each genomic region. The dashed line in the middle of each panel separates the top and bottom strands of DNA. Gene models are drawn as green arrows above and below this line, and clicking on a gene will cause its annotation to appear in a box.
The pink blocks in these panels are genomic regions identified by BlastZ as being similar in sequence composition. If you click on a pink block, a transparent wedge is drawn connecting it to its partner region in the other genome, and information about the blast hit (also known as an HSP) is shown in an information box. You can click on all the pink blocks individually to connect every region of sequence similarity, or hold the "shift" key and then click on one of the pink blocks to connect them all at once. Since we are analyzing "breaks" in our dotplot that are the result of an insertion or deletion, we expect to see at least one genomic region that is present in one genome and not the other. These indels will be evident by the pattern of transparent wedges and pink blocks.
This corresponds to the GEvo analysis of the first break in our dotplot. You can regenerate the high resolution analysis here. Click on all the pink blocks in order to connect every region of sequence similarity, or hold the shift key when clicking on a blast hit to connect every region at once. Note that deletion in DH10B creates a gap between the transparent wedges. Click on individual genes in REL606 to identify which genes are missing in DH10B. We can conclude that this is an example of deletion because of presence of pseudogenes at site of deletion in DH10B. Perhaps transposon(s) insertion created pseudogenes that later got deleted. Remember we are looking at these genomes at a single time point and trying to trace back its history. Based on this fact, we can hypothesize that at some point in past, tranposon(s) had integerated into what is now a site of deletion in DH10B. As we will see that transposition is the most common cause of changes introduced in genomes and so it is most likely that this deletion in DH10B was the result of transposon insertion.
When you click on the dotplot, GEvo will display 50000 nucleotides towards the left and right from the anchor position. In order to view genes at a high resolution, use side bars to restrict the region of genome being displayed. This will magnify the region of interest and allows to view minute details. This is particularly helpful when finding direct repeats to account for insertions.
High-resolution GEvo analysis of an insertionInsertions in bacterial genome could be from exogenous DNA such as plasmids and phages or it could be the result of transposition or translocation. Let us consider the third break in our dotplot. Run GEVo analysis. Notice that on the edges of "inserted" genes in REL606, the pink wedges overlap at a common syntenic region in DH10B. These genes are apparently missing in DH10B at this particular locus. Notice that the information box that appears with connectors show almost the same percent identity for both ends ~99%. These are direct repeats.
Click on the inserted genes to view their annotations. There is a lac operon and few other metabolic genes. But how can a critical operon be missing from E-coli DH10B? If you go back to our dotplot and align the locator on third break, you will see a fragment of green line right above the break. Position the locator on it and click. GEvo analysis will show that the region of "inserted" genes in REL606 is syntenic to region on a farther locus in DH10B. You will see lac operon and other metabolic genes which are missing in DH10B at third break are present elsewhere on its genome. This is an example of translocation. In this case syntenic regions are not collinear are are located at different positions in their respective genomes.
Let us consider the ninth break in the dotplot. Run GEvo Analysis for this break. Magnify the region from 50k to 100k using side bars. Notice that a number of flagella genes are present in DH10B but are missing in REL606. The presence of direct repeats is not evident yet and we cannot conclude that insertion happened in DH10B. We will BLAST DH10B against itself to see any direct repeats. If this is indeed an insertion, then we expect to see color coded bars bordering the DNA region containing these flagella genes in DH10B. Click on "Add sequence" and copy and paste the gene ID of DH10B into "name" box in the third sequence. This option allows us to view multiple sequences at the same time. Adjust the number of sequences on left and right to view syntenic regions on all three sequences.
These are direct repeats and evidence for insertion in DH10B. Direct repeats allows us to infer that there was a staggered cut in the DNA of DH10B followed by insertion of linear piece of DNA. The gaps at the ends were repaired and ligated. These repaired gaps became direct repeats that flank the DNA segment containing flagellar genes. Notice that there is not much support that this is a translocation. You can click on several green dots that align with ninth break on our dotplot. These regions are mostly repeats which are syntenic all over the genome and are therefore "noise" in our data.Next, we will look at phage insertion. Consider the second break in the dotplot. Run GEVo analysis on this break. Before checking for direct repeats, determine the identity of genes inserted in DH10B. These are phage-specific genes. CP4-6 prophage has integrated its DNA at this locus as seen by CP4-6 specific integrase, DNA binding protein etc.
Next we will look at an example of inversion. Consider the twelfth break on the dotplot. Run GEvo Analysis on this break. Notice the syntenic regions between 10K and 30K on DH10B. Click on the pink blocks and notice the pattern of transparent wedges that connect the syntenic regions. These genes are inverted. Notice IS10R and IS10L bordering the inverted region. These IS elements have created inverted terminal repeats (ITR) which tend to invert or flip the genes within them.
Detailed analysis of each syntenic discontinuity
| Variation type
|| Difference in strain B REL606
|| Difference in strain K-12 DH10B
|| Link leading to GEvo |
| 1. Deletion
|| Deletion of ~18 genes including DNA
pol II, genes in metabolic pathway, thiamine ABC transporter
| pseudogenes in DH10B at deletion site.
|| Possible additional insertion in DH10B as evidenced by
pseudogenes of yabP, RNA pol associated helicase and FruR, that are not present in REl606
| 2. Insertion
|| Insertion of IS1 transposon
||Insertion sequences and Prophage CP46 DNA insertion|| Prophage specific genes found in DH10B
|| Prophage DNA insertion and IS insertions has created pseudogenes in K-12 DH10B
| 3. Translocation in REL606 and insertion in DH10B
||Insertion of IS1 sequence. Translocation of ~15 genes including lac operon and other metabolic enzymes genes||Insertion of IS3 and IS2 sequences||
Translocation in REL606 as evidenced by direct repeats.Dotplot shows that the missing genes are present in DH10B but not in this locus. The syntenic region is therefore not colinear.
Pseudogenes of yaiT and yaiX were created in DH10B by transposon insertions.
Insertion by translocation in REL606 was confirmed as lac operon and other metabolic genes were found in DH10B by analyzing the translocated genes on the dotplot
| 4. Insertion in REL606 and DNA duplication event in DH10B.
|| Prophage DNA and transposase insertion
||Recent DNA duplication event|| 100% identity between paralogs in DH10B and ~98% identity between syntenic region of DH10B and REL606
|| Possible phage DNA insertion in REL606 as "hypothetical protein" genes were found near putative prophage tail component gene in REL606.
| 5. Insertion
|| Bacteriophage DNA insertion
|| IS2 sequence insertion
|| Pseudogenes at IS2 insertion site in DH10B. Phage specific genes were found in REL606
|| Possible phage DNA insertion in REL606 as "Hypothetical proteins" were found near phage specific genes
| 6. Insertion
|| Prophage DNA insertion
|| Phage specific genes were found in REL606
| 7. Insertion,translocation and inversion
||none||Prophage DNA insertion and translocation of nitrite reductase 2 genes|| Phage specific genes found in DH10B
|| Translocation in DH10B is evident by dotplot. Moreover, the translocated genes in DH10B were found to be inverted. It could not be determined genes on which genomes were inverted as tranposon insertions were found in both genomes.
| 8. Insertion and deletion
|| Transposon insertions and deletion of phenylacetic acid degradation genes
||IS and Rac prophage DNA insertion|| Phage specific genes found in DH10B. IS or transposon insertions in REL606 might have created direct repeats and facilitated excision of phenylacetic acid degradation genes.
||Rac prophage DNA disrupted by transposon insertion in DH10B|| tinyurl.com/yccbmsq|
|9a. Insertion||9a. none||9a. Insertion of IS5 sequence||9a. none||9a. none||9a. http://tinyurl.com/ylllc6u|
|9b. Insertion||9b. Insertion of ISI transposon||9b. none||9b. none||9b. none||9b. tinyurl.com/ygsqg2f|
|9c. Insertion|| 9c. ISI insertion
||9c. Insertion of ABC transporter, flagella encoding genes and few other enzymes|| 9c. Inserted DNA segment in DH10B is bordered by direct repeats at both ends. 100% identity was found between the two repeats.
||9c.DR indicates transposon insertion in DH10B.||9ctinyurl.com/yza4jy3|
|9d. Insertion|| 9d. IS2 insertion
||9d. none||9d. none||9d. none||9d. tinyurl.com/ygfgtqy|
| 10. Insertion and deletion
|| Bacteriophage DNA insertion and IS1 transposon insertion. Deletion of ~5 genes
|| Insertion of IS3
||IS1 insertion at the site of deletion. Another IS1 insertion might have created direct repeats and facilitated deletion.||none|| tinyurl.com/ykynub2|
| 11. Insertion and deletion
|| IS1 insertion
|| CP4-57 prophage DNA insertion and possible deletion of ParB family protein and recombinase
|| Phage insertion in DH10B may have created pseudogene of ParB family protein genes and recombinase which later got deleted
|| Pseudogenes of yqa, yga and ypj indicated possible formation of pseudogenes of ParB and recombinase at some time prior to their deletion in DH10B.
| 12. Insertion, deletion and Inversion
||IS1 insertion.||IS5 and IS10 transposon insertion. Inversion of ornithine decarboxylase, M-type protein and bifunctional prepilin peptidase/methylase. Deletion of saframycin synthetase, capsule related genes, bio-film formation genes, anti-toxin system and type II secretory apparatus genes.||
Inversion in DH10B as evidenced by inverted repeats of IS10 transposon.
Deletion of several genes in DH10B is evidenced by IS5 trans-activator transposase and presence of pseudogenes in DH10B,
Insertion of IS5 trans-activator transposase indicates possible deletion of several genes in DH10B. Also, no evidence of insertion in REL606 was found such as direct repeats.
13a. Deletion of putative adhesin
|13a. No direct repeats were found to indicate insertion of putative adhensin in REL606 therefore deletion in DH10B may have happened|| 13a.none
|13b. IS1 insertion and deletion of lipopolysaccharide genes||
||13b. IS1 insertion created pseudogene.||13b.http://tinyurl.com/yj2yg5s|
13c. Insertion of IS30 transposon and several 'hypothetical protein" genes.
13c. Insertion in REL606 is evidenced by direct repeats
13c. direct repeats were found in REL606 which indicates insertion of ShiA-like and TrbC-like genes.
|14a. Insertion of several transposons and secondary glycine betaine transporter||14a.Insertion of several transposons. Insertion of Kple2 phage-like element||14a. Direct repeats bordering secondary glycine betaine transporter indicates its insertion||14a. none||14a. tinyurl.com/yzyvunx|
|14b. Insertion||14b. Insertion of ~15 genes||14b. Phage insertion. Transposon insertions||14b. Insertion in REL606 is evidenced by direct repeats flanking the DNA segment containing several genes.||14b.Phage-like genes were found in DH10B||14b. tinyurl.com/yly2b6u|
14c. Deletion of ~15 genes.
14c. Deletion in DH10B is evidenced by insertion of IS10R which may have facilitated excision of DNA by forming direct repeats
14c. Pseudogenes found at the site of deletion and IS10R insertion.