Install coge

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Installing CoGe on a blank Ubuntu machine.

Initial Dependencies

Run the following command: 

sudo apt-get -y install {program}


where {program} is each of the following: 

git
mysql-server
samtools
ubuntu-dev-tools
build-essential checkinstall
gcc-multilib
expat libexpat1-dev
libgd2-xpm-dev build-essential
njplot
imagemagick
graphviz
apache2
lamp-server^
phpmyadmin
swig
ttf-mscorefonts-installer
python-setuptools
python-numpy
python-dev
aragorn
python-software-properties

After python-software-properties is installed the following additional packages need be added: 

sudo apt-add-repository ppa:chris-lea/node.js
sudo apt-get update
sudo apt-get install node 
sudo npm install -g bower

Then, use: 

sudo cpan App::cpanminus
sudo cpanm {module}

where {module} is each of the following: 

AuthCas
DBIx::Class
DBIx::Class::Schema
DBD::SQLite
Data::UUID
Data::GUID
XML::SAX::Expat
XML::Parser
XML::Simple
IPC::System::Simple
CGI::Application::Dispatch
CGI::Log
GD::Graph::bars
Moose
ZMQ::LibZMQ3
File::Slurp
File::Touch
DateTime
URI::Split
JSON
JSON::XS

If cpanm is being troublesome, try 

cpanm --reinstall

Create new mysql database

Dump CoGe Database schema 

mysqldump -d -h localhost -u root -pXXXXXXX coge | sed 's/AUTO_INCREMENT=[0-9]*\b//' > cogetable.sql

Note: be sure to disable apparmour for MySQL.

Create new CoGe Database 

create database coge

Initialize new coge database 

mysql -u root -pXXXXXXXX coge < cogetable.sql

Populate a few entries in the feature_type table 

mysql -u root -pXXXXXXXXX coge < feature_type.sql

Create new user for new CoGe database

  • Want a web-user with limited write privileges and a power user to load new data
create user 'coge'@'localhost' IDENTIFIED BY 'XXXXXX';
grant all privileges on coge.* to coge;
create user 'coge_web'@'localhost' IDENTIFIED BY 'XXXXXX';
grant select on coge.* to coge_web;
flush privileges;

Note: The CoGe web-user needs edit/insert permission on some tables. Here is a snapshot of what these are:

Deploy new CoGe Web-site

Generate an RSS key 

ssh-keygen
cd .ssh

Copy the contents of the public key into Github, and then download the CoGe repository 

git clone https://github.com/LyonsLab/coge.git

Make directories where files go and give write persmission 

cd coge/web
./setup.sh


Configure apache

The /etc/apache2/sites-available/default.conf should look like this:

<VirtualHost *>
	ServerAdmin webmaster@localhost
	
	DocumentRoot /opt/coge/web
	<Files ~ "\.(pl|cgi)$">
    		SetHandler perl-script
    		PerlResponseHandler ModPerl::Registry
    		Options +ExecCGI
    		PerlSendHeader On
	</Files>

	<Directory />
		Options FollowSymLinks
		AllowOverride None
	</Directory>
	<Directory /opt/coge/web>
		PerlSetEnv COGE_HOME "/opt/coge/web"
		Options Indexes FollowSymLinks MultiViews
		AllowOverride None
		Order allow,deny
		allow from all
		# This directive allows us to have apache2's default start page
                # in /apache2-default/, but still have / go to the right place
                #RedirectMatch ^/$ /apache2-default/
	</Directory>
	
	<Directory /opt/coge/web/services/>
        	Options +FollowSymLinks +ExecCGI
        	AddHandler wsgi-script .py
    	</Directory>

    	<Directory /opt/coge/web/services/JBrowse/JBrowse_TrackContent_WS/>
        	Options +FollowSymLinks +ExecCGI
        	AddHandler wsgi-script .py
    	</Directory>
    	Alias /CoGe/jex /opt/coge/web/services/jex.py
    	Alias /CoGe/services/JBrowse/track /opt/coge/web/services/JBrowse/J$
    	Alias /CoGe "/opt/coge/web"

	Alias /gobe/ /opt/coge/web/gobe/
	<Directory "/opt/coge/web/gobe/">
		Options +FollowSymLinks +ExecCGI
		AddHandler wsgi-script .py
	</Directory>
	
	ScriptALias /api/v1/jbrowse/ /opt/coge/web/services/service.pl
	Alias /services/JBrowse/track /opt/coge/web/services/JBrowse/JBrowse_TrackContent_WS/source.py
	ScriptAlias /api/v1/ /opt/coge/web/services/mojolicious/
	ScriptAlias /cgi-bin/ /opt/coge/web/
	<Directory "/opt/coge/web">
		AllowOverride None
		Options +ExecCGI -MultiViews +SymLinksIfOwnerMatch
		AddHandler cgi-script .pl
		Order allow,deny
		Allow from all
	</Directory>

	wsgiScriptAlias /jex /opt/coge/web/services/jex.py

	ErrorLog /var/log/apache2/error.log

	# Possible values include: debug, info, notice, warn, error, crit,
	# alert, emerg.
	LogLevel warn

	CustomLog /var/log/apache2/access.log combined
	ServerSignature On

    Alias /doc/ "/usr/share/doc/"
    <Directory "/usr/share/doc/">
        Options Indexes MultiViews FollowSymLinks
        AllowOverride None
        Order deny,allow
        Deny from all
        Allow from 127.0.0.0/255.0.0.0 ::1/128
    </Directory>

</VirtualHost>


Configure coge.conf file

Replacing XXX's with your own information. (Change paths as necessary; this template is configured for having the Coge directory in the path: /opt/coge)

##This is a configuration file for CoGe.

#database configuration
DBNAME	  coge
DBHOST	  localhost
DBPORT	  3307
DBUSER	  coge
DBPASS	  XXXXXXX

#CAS authentication for webservices
CAS_URL https://auth.iplantcollaborative.org/cas

#basic auth name and password
AUTHNAME XXXXXX
AUTHPASS XXXXXX

#web cookie name
COOKIE_NAME cogec

#support email address
SUPPORT_EMAIL XXXXXX

#basedir for coge
COGEDIR	    /opt/coge/web/

#bin dir for coge's programs
BINDIR	    /opt/coge/web/bin/

#scripts dir for coge's programs
SCRIPTDIR	/opt/coge/scripts

RESOURCESDIR /opt/coge/resources

#data dir for coge's programs
DATADIR	    /storage/coge/data/

#cache dir
CACHEDIR /storage/coge/data/cache/

#dir for pair-wise whole genome comparisons (e.g. SynMap)
DIAGSDIR	    /opt/coge/web/data/diags/

#fasta dir
FASTADIR	    /opt/coge/web/data/fasta/

#sequence dir
SEQDIR	    /storage/coge/data/genomic_sequence/
#SEQDIR	   /opt/tmp/data/

#experiment dir
EXPDIR  /storage/coge/data/experiments/

#TMPL dir for CoGe's web page templates
TMPLDIR	    /opt/coge/web/tmpl/

#temp dir for coge
TEMPDIR	  /opt/coge/web/tmp/

#secure temp dir
SECTEMPDIR /storage/coge/tmp/

#IRODS dir
IRODSDIR        /iplant/home/<USER>/coge_data
IRODSENV        /opt/coge/web/irodsEnv

#Base URL for web-server
URL	    /

#URL for temp directory
TEMPURL	    /tmp/

#blast style scoring matrix dirs
#BLASTMATRIX    /opt/coge/web/data/blast/matrix/
BLASTMATRIX    /storage/coge/data/blast/matrix/

#blastable DB
#BLASTDB    /opt/coge/web/data/blast/db/
BLASTDB    /storage/coge/data/blast/db/

#lastable DB
#LASTDB    /home/franka1/coge/web/data/last/db/
LASTDB    /storage/coge/data/last/db/

#directory for bed files
BEDDIR	   /opt/coge/web/data/bed/

#servername for links
SERVER     http://XXXXXX/

#Job Engine Server
JOBSERVER localhost

#Job Engine Port
JOBPORT 5151

#directory for caching genome browser images
IMAGE_CACHE /opt/coge/web/data/image_cache/

#maximum number of processor to use for multi-CPU systems
MAX_PROC 32
COGE_BLAST_MAX_PROC 8

#True Type Font
FONT	/usr/share/fonts/truetype/msttcorefonts/arial.ttf

#SynMap workflow tools
KSCALC /opt/coge/web/bin/SynMap/kscalc.pl
GEN_FASTA /opt/coge/web/bin/SynMap/generate_fasta.pl
RUN_ALIGNMENT /opt/coge/web/bin/SynMap/quota_align_merge.pl
RUN_COVERAGE /opt/coge/web/bin/SynMap/quota_align_coverage.pl
PROCESS_DUPS /opt/coge/web/bin/SynMap/process_dups.pl
GEVO_LINKS /opt/coge/web/bin/SynMap/gevo_links.pl
DOTPLOT_DOTS /opt/coge/web/bin/dotplot_dots.pl

#various programs
BL2SEQ	 /usr/local/bin/legacy_blast.pl bl2seq
BLASTZ	 /usr/local/bin/blastz
LASTZ	 /usr/local/bin/lastz
MULTI_LASTZ	/opt/coge/web/bin/blastz_wrapper/blastz.py
LAST_PATH	/opt/coge/web/bin/last_wrapper/
MULTI_LAST	/opt/coge/web/bin/last_wrapper/last.py
#BLAST 2.2.23+
BLAST	 /usr/local/bin/legacy_blast.pl blastall
TBLASTN  /usr/local/bin/tblastn
BLASTN	 /usr/local/bin/blastn
BLASTP	 /usr/local/bin/blastp
TBLASTX	 /usr/local/bin/tblastx
FASTBIT_LOAD  /usr/local/bin/ardea
FASTBIT_QUERY /usr/local/bin/ibis
SAMTOOLS /usr/bin/samtools
RAZIP /usr/local/bin/razip

###Formatdb needs to be updated to makeblastdb
FORMATDB /usr/bin/formatdb
LAGAN	 /opt/coge/web/bin/lagan-64bit/lagan.pl
LAGANDIR /opt/coge/web/bin/lagan-64bit/
CHAOS	 /opt/coge/web/bin/lagan-64bit/chaos
GENOMETHREADER /opt/coge/web/bin/gth
DIALIGN	       /opt/coge/web/bin/dialign2_dir/dialign2-2_coge
DIALIGN2	       /opt/coge/web/bin/dialign2_dir/dialign2-2_coge
DIALIGN2_DIR	       /opt/coge/web/bin/dialign2_dir/
HISTOGRAM	       /opt/coge/web/bin/histogram.pl
KS_HISTOGRAM	       /opt/coge/web/bin/ks_histogram.pl
PYTHON		       /usr/bin/python
PYTHON26		   /usr/bin/python
DAG_TOOL	       /opt/coge/web/bin/SynMap/dag_tools.py
BLAST2BED	       /opt/coge/web/bin/SynMap/blast2bed.pl
TANDEM_FINDER	       /opt/coge/web/bin/dagchainer/tandems.py
DAGCHAINER	       /opt/coge/web/bin/dagchainer_bp/dag_chainer.py
EVALUE_ADJUST	       /opt/coge/web/bin/dagchainer_bp/dagtools/evalue_adjust.py
FIND_NEARBY	       /opt/coge/web/bin/dagchainer_bp/dagtools/find_nearby.py
QUOTA_ALIGN	       /opt/coge/web/bin/quota-alignment/quota_align.py
CLUSTER_UTILS	       /opt/coge/web/bin/quota-alignment/cluster_utils.py
BLAST2RAW	       /opt/coge/web/bin/quota-alignment/scripts/blast_to_raw.py
SYNTENY_SCORE	       /opt/coge/web/bin/quota-alignment/scripts/synteny_score.py
DOTPLOT	       /opt/coge/web/bin/dotplot.pl
SVG_DOTPLOT	       /opt/coge/web/bin/SynMap/dotplot.py
NWALIGN	       /usr/local/bin/nwalign
CODEML	       /opt/coge/web/bin/codeml/codeml-coge
CODEMLCTL      /opt/coge/web/bin/codeml/codeml.ctl
CONVERT_BLAST  /opt/coge/web/bin/convert_long_blast_to_short_blast_names.pl
DATASETGROUP2BED	/opt/coge/web/bin/dataset_group_2_bed.pl
ARAGORN			/usr/local/bin/aragorn
CLUSTALW		/usr/local/bin/clustalw2
GZIP	/bin/gzip
GUNZIP	/bin/gunzip
TAR	/bin/tar

#MotifView
MOTIF_FILE /opt/coge/web/bin/MotifView/motif_hash_dump

#stuff for Mauve and whole genome alignments
MAUVE	/opt/coge/web/bin/GenomeAlign/progressiveMauve-muscleMatrix
COGE_MAUVE	/opt/coge/web/bin/GenomeAlign/mauve_alignment.pl
MAUVE_MATRIX /opt/coge/web/data/blast/matrix/nt/Mauve-Matrix-GenomeAlign
#newicktops is part of njplot package
NEWICKTOPS		/usr/bin/newicktops
#convert is from ImageMagick
CONVERT			/usr/bin/convert
#from graphviz
DOT
NEATO


CUTADAPT /usr/local/bin/cutadapt
GSNAP /usr/local/bin/gsnap
CUFFLINKS /usr/local/bin/cufflinks
PARSE_CUFFLINKS /opt/coge/scripts/parse_cufflinks.py
GMAP_BUILD /usr/local/bin/gmap_build
BOWTIE_BUILD /usr/local/bin/bowtie2-build
TOPHAT /usr/local/bin/tophat

#THIRD PARTY URLS
GENFAMURL http://dev.gohelle.cirad.fr/genfam/?q=content/upload
GRIMMURL http://grimm.ucsd.edu/cgi-bin/grimm.cgi#report
QTELLER_URL http://geco.iplantc.org/qTeller

Install Perl Modules and other remaining dependencies

  • Install CoGe modules /modules directory of CoGe install path
sudo perl Makefile.PL lib=/usr/local/lib/perl/5.18.2; sudo make install
  • Install the javascript dependencies from the root install path
bower install
  • Each .pm file in /coge/web will have a list of perl modules at the top of the file. Use 'sudo cpanm' to install these.
  • For each path in coge.conf that starts with /usr/local/bin, download these programs and follow the installation instructions on their respective websites. 
  1. GMAP Gsnap, and fastbit (be sure to get version 1.3.5, rather than the most recent) require the command './configure && make && sudo make install'
  2. For clustalw, cufflinks, TopHat, and Bowtie (needs unzipping), copy the executable files into /usr/local/bin
  3. Nwalign and cutadapt need the command 'sudo python setup.py install'

After installing modules use 

sudo service apache2 restart

to reset the Apache server and allow for proper testing.

Install JBrowse

Download and install the JBrowse package from http://jbrowse.org/install/ 

cd js
unzip JBrowse-1.11.4-dev.zip
mv JBrowse-1.11.4 /coge/web/js/jbrowse

Install CCTools

  • Extract the file (this example is using version 4.1.3 which may differ from the version downloaded)
tar xzvf cctools-4.1.3-source.tar.gz
  • Compile and install
cd cctools-4.1.3-source
./configure --prefix /usr/local
make
make install
  • Add the following upstart scripts for the work_queue_pool and catalog_server to /etc/init

By default the pool directory for work_queue will be in /storage/work_queue adjust the directory as needed. 

# /etc/init/work_queue_pool.conf

description "The cctools work queue pool"
author "Evan Briones"

start on (local-filesystems and net-device-up IFACE=eth0)

stop on shutdown

respawn limit 30 60

pre-start script
    POOL_DIR=/storage/work_queue
    LOG_FILE=$POOL_DIR/logs/work_queue_pool.log

    # Add the pool directory and set ownership
    if ! [ -d "$WORK_DIR" ]; then
        mkdir -p $POOL_DIR/workers
        mkdir -p $POOL_DIR/logs
        chown -R www-data:www-data $POOL_DIR
    fi

    # Remove the pidfile if it exists
    rm -f $POOL_DIR/work_queue_pool.pid

    # Archive old log and timestamp the value
    if [ -f "$LOG_FILE" ]; then
        TIMESTAMP=$(date  +"%Y-%m-%d.%H.%m.%S")
        mv -f $LOG_FILE "$LOGFILE.$TIMESTAMP"
    fi
end script

script
    POOL_DIR=/storage/work_queue
    LOG_FILE=$POOL_DIR/logs/work_queue_pool.log
    CONFIG=/etc/yerba/work_queue_pool.conf
    WORK_QUEUE_POOL=$(which work_queue_pool)

    export CATALOG_HOST=localhost
    export CATALOG_PORT=1024

    exec start-stop-daemon -c www-data -g www-data -d $POOL_DIR --start \
        -p $POOL_DIR/work_queue_pool.pid --exec $WORK_QUEUE_POOL \
        -- -M coge-.* -d all -o $LOG_FILE -w 10 \
        -S $POOL_DIR -E --workdir=$POOL_DIR/workers
end script

# /etc/init/catalog_server.conf

description "The cctools catalog server"
author "Evan Briones"

start on (local-filesystems and net-device-up IFACE=eth0)

stop on shutdown

respawn

script
    exec catalog_server -p 1024 -l 100 -T 3
end script
  • Start the catalog server and work_queue_pool
sudo start work_queue_pool
sudo start catalog_server

Install Yerba

Download and install the latest Yerba package from https://github.com/LyonsLab/Yerba/archive/v0.3.4.tar.gz

The default installation path for Yerba will be in /opt/Yerba. If another path is chosen update the configuration files to match. 

# /etc/init/yerba.conf

description "Yerba server daemon"
author "Evan Briones"

start on (local-filesystems and net-device-up IFACE=eth0
    and catalog_server and work_queue_pool)

stop on shutdown

respawn

pre-start script
    LOG_DIR=/opt/Yerba/log
    LOG_FILE=$LOG_DIR/debug.log
    [ -d "$LOG_DIR" ] || mkdir -m777 -p $LOG_DIR
#    [ -f "$LOG_FILE" ] || rm -f $LOG_FILE
end script

script
    export YERBA_ROOT=/opt/Yerba
    export PYTHONPATH="/usr/local/lib/python2.7/site-packages:$YERBA_ROOT"
    exec start-stop-daemon -c www-data -g www-data --start \
        --iosched real-time --nicelevel -1 \
        --exec $YERBA_ROOT/bin/yerbad -- >> $YERBA_ROOT/log/debug.log 2>&1
end script

Populate with test data

scripts/replicate_genome_between_coge_installations.pl -dsgid 11022 -u1 coge -p1 XXXXXXX -db1 coge -u2 oryza_coge -p2 XXXXXXX -db2 oryza_coge -sd /opt/apache/oryz_coge/data/genomic_sequence

Troubleshooting

Visualization in GEvo does not work

This relies on a system known as Gobe. Check the following things:

  • Apache configuration for gobe
  • Check to see if paths hard-coded into gobe/flash/service.wsgi need to be updated
    • NOTE: Not sure if this is required


Working on an Atmosphere Virtual Machine

Click here for instructions on dealing with issues that occur specifically with Atmosphere Virtual machines.

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