Difference between revisions of "SynFind"

Revision as of 15:38, 23 May 2012

Overview

SynFind identified syntenic regions against any set of genomes given a gene in one genome. The primary algorithm of this analysis is SyntenyScore written by Haibao Tang.

Syntenic Depth

SynFind calculates the number and percentage of genes for a particular Syntenic depth in each target genome.

SynFind Syntenic Depth Examples

Options for SynFind

General parameters

• Comparison algorithm: Select the algorithm for comparing genomes. We recommend Last! Much, much faster than LastZ!

Synteny Finding: SyntenyScore

SynFind is powered by SyntenyScore (part of the BaoTools Package)

Parameters

• Gene window Size: synteny window size in genes [default: 40]
  • The Window Size is the size of the genomic regions compared between two genomes using genes as the metric. Given an anchor gene, the window size is divided by 2 and that many genes searched up and downstream from the anchor.
  • Example, a window size of 40 means that a total of 41 genes are checked: the anchor gene; plus 20 upstream; plus 20 downstream.
• Minimum number of genes: The minimum number of anchoring genes to call a region syntenic. [default: 4]
• Scoring Function: scoring scheme, must be one of ('collinear', 'density') [default: collinear]
  • Collinear: a collinear arrangement of syntenic genes in enforced
  • Density: any arrangement of gene-pairs is tolerated

Reference on Scoring Method

Different Gene Families in Arabidopsis thaliana Transposed in Different Epochs and at Different Frequencies throughout the Rosids

Margaret R. Woodhouse, Haibao Tang, Michael Freeling

http://www.plantcell.org/content/23/12/4241.abstract

Download Synteny Score code

• Full github repo: https://github.com/tanghaibao
• Package with SyntenyScore: https://github.com/tanghaibao/quota-alignment
• Synteny_Score only: https://github.com/tanghaibao/quota-alignment/blob/master/scripts/synteny_score.py