Difference between revisions of "Genome masking"

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** CoGe's visualizations often use purple to represent genome sequence "X" to make the identification of masked reasons easy
 
** CoGe's visualizations often use purple to represent genome sequence "X" to make the identification of masked reasons easy
  
Note:  We will (eventually) have an option in CoGe that allows people to mask their own genomes using this pipeline.  Until that is available, please [[mailto:coge.genome@genome.com email the coge team]] with the [[genome id]] of the genome you would like masked.
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For users that are logged in, CoGe provides an option to mask a genome through [[GenomeInfo]]
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[[File:Screen Shot 2016-02-01 at 9.20.58 AM.png]]

Latest revision as of 10:22, 1 February 2016

CoGe masks genomes by using NCBI's windowmasker: http://www.ncbi.nlm.nih.gov/pubmed/16287941

Currently, our pipeline for masking is:

  • Generate frequency counts for the masking database for the genome
    • windowmasker -in <genome> -mk_counts -out <counts>
  • Mask genome
    • windowmasker -in <genome> -ustat <counts> -outfmt fasta -dust T -out <masked genome>
  • Convert soft masking (lower case sequence data) to "X".
    • CoGe's visualizations often use purple to represent genome sequence "X" to make the identification of masked reasons easy

For users that are logged in, CoGe provides an option to mask a genome through GenomeInfo

Screen Shot 2016-02-01 at 9.20.58 AM.png