Tomato-Utricularia Syntenic Depth

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Syntenic depth measurements permit classifying the number of whole genome duplications that have happened in one lineage relative to another. Unfortunately, these analyses are not 100% conclusive, but provide supporting evidence, especially when combined with Syntenic dotplots.

Using v4 of the Utricularia genome (http://genomevolution.org/CoGe//OrganismView.pl?dsgid=19456)

4 genes in an 80 gene window

SynFind: http://genomevolution.org/r/4tqx

Utricularia gibba

Depth 1	6972	of	19459	35.83%
Depth 2	4727	of	19459	24.29%
Depth 3	3392	of	19459	17.43%
Depth 4	2153	of	19459	11.06%
Depth 5	1230	of	19459	6.32%
Depth 6	603	of	19459	3.10%
Depth 7	223	of	19459	1.15%
Depth 8	118	of	19459	0.61%
Depth 9	39	of	19459	0.20%
Depth 10	2	of	19459	0.01%

4 genes in a 40 gene window

SynFind: http://genomevolution.org/r/4tqi

Utricularia gibba

Depth 1	5566	of	8468	65.73%
Depth 2	2071	of	8468	24.46%
Depth 3	664	of	8468	7.84%
Depth 4	137	of	8468	1.62%
Depth 5	25	of	8468	0.30%
Depth 6	5	of	8468	0.06%

Materials and Methods

Syntenic Depth tables are calculated by CoGe's tool SynFind. These analyses used Last [1] to compare protein coding sequences (default parameters) of these genomes and Synteny_Score (of the TangTools [2] software package) to identify syntenic regions (gene window size =80, minimum number of genes =4; collinear scoring function).


References

  1. Kiełbasa, S. M., Wan, R., Sato, K., Horton, P., and Frith, M. C. (2011). "Adaptive Seeds Tame Genomic Sequence Comparison". Genome Res 21: 487-493. 
  2. Haibao Tang. TangTools. https://github.com/tanghaibao/quota-alignment.